FPSC (Federal Public Service Commission) · Lecturer / Assistant Professor Pharmacy
Radioisotopes and Autoradiography
Biological Techniques
· Biology
13 MCQs
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1
Which radioisotope is commonly utilized in the medical detection of tumors?
Correct Option
Option C
Explanation
Cobalt-60 is a radioactive isotope that emits gamma rays. It is widely used in medical applications, particularly in radiotherapy for the treatment and detection of cancerous tumors due to its high-energy radiation output.
2
How does the concentration of radioactive isotopes in sediment layers change over time, and what is its significance in radiometric dating?
Correct Option
Option A
Explanation
Radiometric dating relies on the principle of radioactive decay, where unstable parent isotopes transform into stable daughter isotopes at a constant, measurable rate. As time progresses, the concentration of the parent isotope decreases exponentially. Therefore, older geological layers contain a lower proportion of the original radioactive parent isotope compared to younger layers, allowing scientists to calculate the age of the sample based on the remaining parent-to-daughter ratio.
3
Match the radioisotopes in List-I with their corresponding diagnostic applications in List-II.
Correct Option
Option C
Explanation
Arsenic-74 is used for tumor detection, Cobalt-60 is used in cancer radiotherapy, Iodine-131 is used to assess thyroid gland activity, and Sodium-24 is used to study blood circulation and disorders. Matching these gives: a-3 (Arsenic-Tumor), b-4 (Cobalt-Cancer), c-1 (Iodine-Thyroid), and d-2 (Sodium-Blood).
4
Why is the radioisotope Cobalt-60 frequently utilized in medical radiation therapy?
Correct Option
Option B
Explanation
Cobalt-60 is a synthetic radioactive isotope used in external beam radiotherapy because it undergoes beta decay and subsequently emits high-energy gamma rays. These gamma rays have high penetrating power, allowing them to target and destroy deep-seated malignant tumor cells while minimizing damage to surrounding healthy tissues. Its stability and predictable decay rate make it a reliable source for clinical oncology applications.
5
Which radioactive isotope is commonly utilized in the medical treatment of certain types of blood cancer?
Correct Option
Option B
Explanation
While Phosphorus-32 is often used for polycythemia vera, Iodine-131 is primarily used for thyroid conditions. The source answer indicates A, though clinical practice varies by specific diagnosis. This question requires careful review regarding the specific isotope application in hematological malignancies.
6
Which of the following medical applications of radioisotopes is incorrectly matched?
Correct Option
Option C
Explanation
Gold-198 is typically used for treating certain cancers, such as prostate or liver tumors, via brachytherapy. It is not standardly used for treating the retina. The other options correctly identify the medical use of the respective radioisotopes.
7
Which radioactive isotope is commonly utilized in medical diagnostics to measure the rate of blood flow within the human circulatory system?
Correct Option
Option A
Explanation
Radioactive sodium (specifically Na-24) is historically used in clinical studies to trace blood circulation and measure blood flow rates. Because sodium ions are readily distributed throughout the plasma, they serve as an effective tracer for monitoring cardiovascular dynamics and peripheral vascular efficiency in medical research and diagnostic procedures.
8
What is the term for the technique where newly synthesized enzymes are labeled using a heavy isotope?
Correct Option
Option B
Explanation
Density labeling is a technique used to distinguish newly synthesized proteins or enzymes from pre-existing ones. By incorporating heavy isotopes (like 15N or 13C) into the growth medium, the new enzymes become denser than the old ones, allowing them to be separated via density gradient centrifugation.
9
Which radioactive isotopes are commonly utilized in the management of blood-related cancers?
Correct Option
Option C
Explanation
Phosphorus-32 is frequently used to treat polycythemia vera, a blood disorder, while Cobalt-60 is a powerful gamma-ray source used in external beam radiotherapy for various malignancies, including certain lymphomas and leukemias. Both isotopes are significant tools in oncology for targeting and destroying cancerous cells through ionizing radiation.
10
Match the substances in List-I with their primary medical or practical applications in List-II.
Correct Option
Option C
Explanation
Atropine is used to dilate the pupil (a-3). Ether is historically used as a local/general anesthetic (b-1). Nitroglycerine is used to treat heart conditions like angina (c-2). Pyrethrin is a natural insecticide used for mosquito control (d-4). The correct sequence is a-3, b-1, c-2, d-4.
11
What is the primary application of Radioimmunoassay (RIA) in a clinical setting?
Correct Option
Option A
Explanation
Radioimmunoassay is a highly sensitive in vitro laboratory technique used to measure concentrations of antigens, such as hormones or antibodies, in biological samples like blood. It utilizes radiolabeled antigens and antibodies to create a competitive binding reaction, allowing for precise quantification of substances present in extremely low concentrations.
12
Which isotope, upon radioactive decay, emits only gamma rays and alpha particles?
Correct Option
Option D
Explanation
The isotope 40U (often referring to Uranium-238 or related isotopes in specific contexts) is known for its decay chain. While the specific decay modes of isotopes like 238U and 232Th are complex, the question identifies 40U as the source of alpha and gamma emissions. Note: 40U is not a standard notation for a common isotope, suggesting a potential nomenclature issue in the source material.
13
How do radioactive isotopes differ from their stable counterparts in terms of atomic structure, despite sharing similar chemical properties?
Correct Option
Option B
Explanation
Radioactive isotopes of an element have the same number of protons and electrons as stable isotopes, meaning they behave identically in chemical reactions. They differ only in the number of neutrons in the nucleus, which results in a different atomic mass.